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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: J Immunol. 2019 Apr 24;202(11):3267–3281. doi: 10.4049/jimmunol.1801466

Figure 6: WT and Timp-1−/− PMN contain similar amounts of Mmp-8 and Mmp-9 and similar quantities of Mmp-8 and Mmp-9 are released from degranulating WT and Timp-1−/− PMNs:

Figure 6:

Extracts of freshly-isolated unstimulated PMNs from WT and Timp-1−/− mice and cell-free supernatant fluids from WT and Timp-1−/− PMN that had been activated with 10−6 M fMLP for 30 min were immuno-blotted for Mmp-9 (A) or Mmp-8 (B). The arrows indicate the proMMP-9 and proMmp-8 forms of the Mmps. Note that extracts of unstimulated WT and Timp-1−/− PMNs contain similar amounts of proMmp-9 and proMmp-8 (Mr = 100 kDa for Mmp-9 and 85 kDa for Mmp-8). In addition, when activated with fMLP, Timp-1−/− PMNs freely release similar amounts of Mmp-8 and Mmp-9 exclusively as the latent proMmp forms.