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. 2020 Jan 2;2(1):25–31. doi: 10.1016/j.bsheal.2019.12.009

Fig. 2.

Fig. 2

Interaction of Ebola VLPs with lipid-rafts at different time points. (A) Purified fluorescent Ebola VLPs (green) were added into Vero cells, and at the indicated time points the cells were stained with the Alexa Fluor® 594 conjugated Cholera Toxin Subunit B (CTB) to label the lipid rafts on cell membrane (red). Then the cells were fixed and visualized under confocal microscope. (B) The graph shows the average number of Ebola VLPs per cell at different time points. The average number of Ebola VLPs associated with each cell was calculated from 100 cells randomly chosen from 5 different views at each time point. * indicates P < 0.05. (C) The graph shows the ratio of Ebola VLPs on cell membrane to Ebola VLPs internalized inside of cells. The ratio was calculated from 100 cells randomly chosen from 5 different views at each time point. * indicates P < 0.05.