Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | STOCK Tg(Gnrh1-cre)1Dlc/J | Jackson Laboratory | Stock #: 021207 RRID:IMSR_JAX:021207 |
|
| Genetic reagent (M. musculus) | B6;129S-Gt(ROSA)26Sortm35.1(CAG-AOP3/GFP)Hze/J | Jackson Laboratory | Stock #: 012735 RRID:IMSR_JAX:012735 |
|
| Genetic reagent (M. musculus) | B6.DBA-Tg(Gnrh1-EGFP)1Phs | Spergel et al., 1999 | MGI:6158458 |
|
| Transfected construct (M. musculus) | AAV2-Retro-hSyn-DIO-hM4D(Gi)-mCherry | OBiO Technology (Shanghai) Corp., Ltd | RRID:Addgene_44362 | |
| Antibody | polyclonal guinea pig anti-GnRH antisera | gift G. Anderson, University of Otago | Cat #GA02 RRID:AB_2721118 |
(1: 5000) |
| Antibody | polyclonal rabbit anti-GnRH antisera | gift G. Anderson, University of Otago | Cat #GA01 RRID:AB_2721114 |
(1: 5000) |
| Antibody | Alexa Fluor 488-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 706-545-148, RRID:AB_2340472 | (1:500) |
| Antibody | Alexa Fluor 594 donkey anti-rabbit antibody | Jackson ImmunoResearch Labs | Cat# 711-585-152, RRID:AB_2340621 | (1:500) |
| Antibody | DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) antibody | Thermo Fisher Scientific | Cat# D3571, RRID:AB_2307445 |
300 nM |
| Antibody | Anti-GFP (chicken polyclonal) | Abcam | Cat# AB13970 RRID:AB_300798 |
(1:8000) |
| Antibody | Anti-Synaptophysin 1 (guinea pig polyclonal) | Synaptic Systems | Cat#101004 RRID:AB_1210382 |
(1:800) |
| Antibody | Anti-Vesicular GABA Transporter (rabbit polyclonal) | Synaptic Systems | Cat#131003 RRID:AB_887869 |
(1:800) |
| Antibody | Anti- Gephyrin (guinea pig polyclonal) | Synaptic Systems | Cat#147318 RRID:AB_2661777 |
(1:800) |
| Antibody | Goat anti-chicken (goat polyclonal, Alexa488-conjugate) | Thermo Fisher Scientific | Cat# A-11039 RRID:AB_2534096 |
(1:200) |
| Antibody | Goat anti-guinea pig (goat polyclonal, biotin-conjugated) | Vector Laboratories | Cat# BA-7000 RRID:AB_2336132 |
(1:200) |
| Antibody | Goat anti-rabbit (goat polyclonal, ATTO647N-conjugated) | Sigma-Aldrich | Cat# 40839 RRID:AB_1137669 |
(1:200) |
| Chemical compound, drug | Beta-Estradiol 3-benzoate | Sigma Aldrich | E8515 | |
| Chemical compound, drug | Beta estradiol | Sigma Aldrich | E8875 | |
| Chemical compound, drug | progesterone | Sigma Aldrich | P0130 | |
| Chemical compound, drug | clozapine-N- oxide | Sigma Aldrich | C0832 | |
| Software, algorithm | GraphPad Prism software | GraphPad Prism | RRID:SCR_002798 | Version 6.01 |
| Software, algorithm | ImageJ image analysis software | ImageJ (https://imagej.net/) |
RRID:SCR_003070 | |
| Software, algorithm | Vaa3D data visualization software | Vaa3D (http://www.vaa3d.org) |
RRID:SCR_002609 |