Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | dome | Flybase:FB2020_01 | FLYB:FBgn 0043903 |
|
| Gene (Drosophila melanogaster) | Hml | Flybase:FB2020_01 | FLYB:FBgn 0029167 |
|
| Gene (Drosophila melanogaster) | Tep4 | Flybase:FB2020_01 | FLYB:FBgn 0031888 |
|
| Gene (Drosophila melanogaster) | CG3902 | Flybase:FB2020_01 | FLYB:FBgn 0036824 |
|
| Gene (Drosophila melanogaster) | Mtpα | Flybase:FB2020_01 | FLYB:FBgn 0041180 |
|
| Gene (Drosophila melanogaster) | Mtpβ | Flybase:FB2020_01 | FLYB:FBgn 0025352 |
|
| Gene (Drosophila melanogaster) | whd | Flybase:FB2020_01 | FLYB:FBgn 0261862 |
|
| Gene (Drosophila melanogaster) | Hnf4 | Flybase:FB2020_01 | FLYB:FBgn 0041180 |
|
| Gene (Drosophila melanogaster) | chm | Flybase:FB2020_01 | FLYB:FBgn 0028387 |
|
| Gene (Drosophila melanogaster) | Gcn5 | Flybase:FB2020_01 | FLYB:FBgn 0020388 |
|
| Gene (Drosophila melanogaster) | AcCoAS | Flybase:FB2020_01 | FLYB:FBgn 0012034 |
|
| Gene (Drosophila melanogaster) | Glut1 | Flybase:FB2020_01 | FLYB:FBgn 0264574 |
|
| Gene (Drosophila melanogaster) | ATPCL | Flybase:FB2020_01 | FLYB:FBgn 0020236 |
|
| Gene (Drosophila melanogaster) | sea | Flybase:FB2020_01 | FLYB:FBgn 0037912 |
|
| Gene (Drosophila melanogaster) | bsk | Flybase:FB2020_01 | FLYB:FBgn 0000229 |
|
| Genetic reagent(Drosophila melanogaster) | dome-GAL4 | BloomingtonDrosophilaStock Center | BDSC:81010; FLYB:FBti0022298; RRID:BDSC_81010 | FlyBase symbol: P{GawB}domePG14 |
| Genetic reagent (Drosophila melanogaster) | Hml-dsRed.Δ | Makhijani et al., 2011 | FLYB:FBgn 0041180 |
FlyBase symbol: P{Hml-dsRed.Δ} |
| Genetic reagent (Drosophila melanogaster) | HmlΔ-GAL4 | Sinenko and Mathey-Prevot, 2004 | FLYB: FBgn 0040877 |
FlyBase symbol: P{Hml-GAL4.Δ} |
| Genetic reagent (Drosophila melanogaster) | Pvf2-lacZ | Choi et al., 2008 | FLYB:FBtp0052107 | FlyBase symbol: P{Pvf2-lacZ.C} |
| Genetic reagent (Drosophila melanogaster) | TepIV-GAL4 | Kyoto Stock Center | DGGR:105442; FLYB:FBti0037434; RRID:DGGR_105442 |
FlyBase symbol: P{GawB}NP7379 |
| Genetic reagent (Drosophila melanogaster) | CG3902-YFP | Kyoto Stock Center | DGGR:115356; FLYB:FBti0143519; RRID:DGGR_115356 |
FlyBase symbol: PBac{566 .P.SVS-1}CG3902CPTI100004 |
| Genetic reagent (Drosophila melanogaster) | Mtpα[KO] | Kyoto Stock Center | DGGR:116261; FLYB:FBal0267653; RRID:DGGR_116261 |
FlyBase symbol: MtpαKO |
| Genetic reagent (Drosophila melanogaster) | Mtpβ[KO] | Kyoto Stock Center | DGGR:116262; FLYB:FBal0267654; RRID:DGGR_116262 |
FlyBase symbol: MtpβKO |
| Genetic reagent (Drosophila melanogaster) | UAS-whd RNAi [KK] | ViennaDrosophila RNAi Center | VDRC:v105400; FLYB:FBti0116709; RRID:FlyBase_FBst0477227 |
FlyBase symbol: P{KK100935}VIE-260B |
| Genetic reagent (Drosophila melanogaster) | OreR | BloomingtonDrosophilaStock Center | BDSC:5; FLYB:FBsn0000277; RRID:BDSC_5 | FlyBase symbol: Oregon-R-C |
| Genetic reagent (Drosophila melanogaster) | w[1118] | BloomingtonDrosophilaStock Center | BDSC:3605; FLYB:FBal0018186;RRID:BDSC_3605 | FlyBase symbol: w1118 |
| Genetic reagent (Drosophila melanogaster) | UAS-Hnf4.miRNA | BloomingtonDrosophilaStock Center | BDSC:44398; FLYB:FBti0152533;RRID:BDSC_44398 | FlyBase symbol: P{UAS-Hnf4.miRNA}attP16 |
| Genetic reagent (Drosophila melanogaster) | UAS-whd RNAi | BloomingtonDrosophilaStock Center | BDSC:34066; FLYB:FBal0263076; RRID:BDSC_34066 | FlyBase symbol: whdHMS00040 |
| Genetic reagent (Drosophila melanogaster) | UAS-FOXO.P | BloomingtonDrosophilaStock Center | BDSC:9575; FLYB:FBtp0017636; RRID:BDSC_9575 | FlyBase symbol: P{UAS-foxo.P} |
| Genetic reagent (Drosophila melanogaster) | Hnf4-GAL4 | BloomingtonDrosophilaStock Center | BDSC:47618; FLYB:FBti0136396; RRID:BDSC_47618 | FlyBase symbol: P{GMR50A12-GAL4}attP2 |
| Genetic reagent (Drosophila melanogaster) | UAS-FUCCI | BloomingtonDrosophilaStock Center | BDSC:55121; RRID:BDSC_55121 | FlyBase symbol: P{UAS-GFP.E2f1.1–230}32; P{UAS-mRFP1.NLS.CycB.1–266}19 |
| Genetic reagent (Drosophila melanogaster) | UAS-mito-HA-GFP | BloomingtonDrosophilaStock Center | BDSC:8442; FLYB:FBti0040803; RRID:BDSC_8442 | FlyBase symbol: P{UAS-mito-HA-GFP.AP}2 |
| Genetic reagent (Drosophila melanogaster) | UAS-chm RNAi | BloomingtonDrosophilaStock Center | BDSC:27027; FLYB:FBal0220716; RRID:BDSC_27027 | FlyBase symbol: chmJF02348 |
| Genetic reagent (Drosophila melanogaster) | UAS-Gcn5 RNAi | BloomingtonDrosophilaStock Center | BDSC:33981; FLYB:FBal0257611; RRID:BDSC_33981 | FlyBase symbol: Gcn5HMS00941 |
| Genetic reagent (Drosophila melanogaster) | UAS-AcCoAS RNAi | BloomingtonDrosophilaStock Center | BDSC:41917; FLYB:FBal0279313; RRID:BDSC_41917 | FlyBase symbol: AcCoASHMS02314 |
| Genetic reagent (Drosophila melanogaster) | UAS-Glut1RNAi | BloomingtonDrosophilaStock Center | BDSC:28645; FLYB:FBal0239561; RRID:BDSC_28645 | FlyBase symbol: Glut1JF03060 |
| Genetic reagent (Drosophila melanogaster) | ATPCL[01466] | BloomingtonDrosophilaStock Center | BDSC:11055; FLYB:FBal0007976; RRID:BDSC_11055 | FlyBase symbol: ATPCL01466 |
| Genetic reagent (Drosophila melanogaster) | sea[EP3364] | BloomingtonDrosophilaStock Center | BDSC:17118; FLYB:FBal0131420; RRID:BDSC_17118 | FlyBase symbol: seaEP3364 |
| Genetic reagent (Drosophila melanogaster) | UAS-bsk[DN] | BloomingtonDrosophilaStock Center | BDSC:6409; FLYB:FBti0021048; RRID:BDSC_6409 |
FlyBase symbol: P{UAS-bsk.DN}2 |
| Genetic reagent (Drosophila melanogaster) | UAS-mCD8::GFP | BloomingtonDrosophilaStock Center | BDSC:5137; FLYB:FBti0180511; RRID:BDSC_5137 | FlyBase symbol: P{UAS-mCD8::GFP.L}2 |
| Genetic reagent (Drosophila melanogaster) | UAS-mCD8::RFP | BloomingtonDrosophilaStock Center | BDSC:27400; FLYB:FBti0115747; RRID:BDSC_27400 | FlyBase symbol: P{UAS-mCD8.mRFP.LG}28a |
| Genetic reagent (Drosophila melanogaster) | U-6;sgRNA-whd-KO | BloomingtonDrosophilaStock Center | BDSC:77066; FLYB:FBal0335953; RRID:BDSC_77066 | FlyBase symbol: whdTKO.GS00854 |
| Genetic reagent (Drosophila melanogaster) | U-6;sgRNA-whd-OE | BloomingtonDrosophilaStock Center | BDSC:68139; FLYB:FBal0337690; RRID:BDSC_68139 | FlyBase symbol: whdTOE.GS00536 |
| Genetic reagent (Drosophila melanogaster) | whd[1] | BloomingtonDrosophilaStock Center | BDSC:441; FLYB:FBal0018515; RRID:BDSC_441 | FlyBase symbol: whd1 |
| Genetic reagent (Drosophila melanogaster) | Hnf4[Δ33] | BloomingtonDrosophilaStock Center | BDSC:43634; FLYB:FBal0240651; RRID:BDSC_43634 | FlyBase symbol: Hnf4Δ33 |
| Genetic reagent (Drosophila melanogaster) | Hnf4[Δ17] | BloomingtonDrosophilaStock Center | BDSC:44218; FLYB:FBal0240650; RRID:BDSC_44218 | FlyBase symbol: Hnf4Δ17 |
| Genetic reagent (Drosophila melanogaster) | tubGAL80[ts20] | BloomingtonDrosophilaStock Center | BDSC:7109; FLYB:FBti0027796; RRID:BDSC_7109 | FlyBase symbol: P{tubP-GAL80ts}20 |
| Genetic reagent (Drosophila melanogaster) | hsFlp | BloomingtonDrosophilaStock Center | BDSC:1929; FLYB:FBti0000784; RRID:BDSC_1929 | FlyBase symbol: P{hsFLP}12 |
| Genetic reagent (Drosophila melanogaster) | Ay-GAL4, UAS-GFP | BloomingtonDrosophilaStock Center | BDSC:4411; FLYB:FBti0012290;FBti0003040RRID:BDSC_4411 | FlyBase symbol: P{AyGAL4}25; P{UAS-GFP.S65T}Myo31DFT2 |
| Antibody | anti-P1 (Mouse monoclonal) | Kurucz et al., 2007 | Cat# NimC1, RRID:AB_2568423 | IF(1:50) |
| Antibody | anti-Pxn (Mouse) | Nelson et al., 1994 | IF(1:400) | |
| Antibody | anti-proPO (Rabbit polyclonal) | Jiang et al., 1997 | IF(1:1000) | |
| Antibody | anti-DE-cadherin (Rat polyclonal) | Developmental Studies Hybridoma Bank | Cat# DE-cad, RRID:AB_2314298 | IF(1:50) |
| Antibody | anti-Ci155(Rat polyclonal) | Developmental Studies Hybridoma Bank | Cat# 2A1, RRID:AB_2109711 |
IF(1:2) |
| Antibody | anti-GFP (Rabbit polyclonal) | Invitrogen | Cat# A-11122, RRID:AB_221569 |
IF(1:100) |
| Antibody | anti-H3 (Rabbit polyclonal) | Cell Signaling Technologies | Cat# 9927, RRID:AB_330200 |
IF(1:400), WB(1:1000) |
| Antibody | anti-H3K9 acetylation (Rabbit polyclonal) | Cell Signaling Technologies | Cat# 9927, RRID:AB_330200 |
IF(1:300), WB(1:1000) |
| Antibody | anti-H4 pan acetylation (Rabbit polyclonal) | Cell Signaling Technologies | Cat# 06–598, RRID:AB_2295074 |
IF(1:500) |
| Chemical compound, drug | Sodium butyrate | EMD Millipore | 19–137 | |
| Chemical compound, drug | Nicotinamide | Sigma-Aldrich | 72345 | |
| Chemical compound, drug | Etomoxir | Cayman Chemicals | Cay11969 | 5 µM |
| Chemical compound, drug | Mildronate | Cayman Chemicals | Cay15997 | 100 µM |
| Chemical compound, drug | L-carnitine hydrochloride | Sigma-Aldrich | C0283 | 100 mM |
| Chemical compound, drug | 2-DG | Sigma-Aldrich | D8375 | 100 mM |
| Chemical compound, drug | Sodium acetate | Sigma-Aldrich | 71196 | 50 mM |
| Chemical compound, drug | 2-NBDG | Invitrogen | N13195 | 0.25 mM |
| Chemical compound, drug | LipidTOX | Molecular Probes | H34477 | 1:1000 |
| Chemical compound, drug | Streptavidin-Cy3 | Molecular Probes | SA1010 | 1:200 |
| Chemical compound, drug | Nile red | Molecular Probes | N1142 | 0.5 ug/mL |
| Chemical compound, drug | DHE (Dihydroxy Ethidium) | Molecular Probes | D11347 | 0.3 µM |
| Sequence-based reagent | Pfk_F | This paper | PCR primers | ATCGTATTTTGGCTTGCCGC |
| Sequence-based reagent | Pfk_R | This paper | PCR primers | CCAGAGAGATGACCACTGGC |
| Sequence-based reagent | Hex_F | This paper | PCR primers | CTGCTTCTAACGGACGAACAG |
| Sequence-based reagent | Hex_R | This paper | PCR primers | GCCTTGGGATGTGTATCCTTGG |
| Sequence-based reagent | whd_F | This paper | PCR primers | GGCCAATGTGATTTCCCTGC |
| Sequence-based reagent | whd_R | This paper | PCR primers | TGCCCTGAACCATGATAGGC |
| Sequence-based reagent | Act5C_F | This paper | PCR primers | ACACATTTTGTAAGATTTGGTGTGT |
| Sequence-based reagent | Act5C_R | This paper | PCR primers | CCGTTTGAGTTGTGCTGT |
| Sequence-based reagent | Mcad_F | This paper | PCR primers | GGCCTGGATCTCGATGTGTT |
| Sequence-based reagent | Mcad_R | This paper | PCR primers | GATCACAGGAGTTTGGCCCAG |
| Sequence-based reagent | Mtpα_F | This paper | PCR primers | ATCACTGTTGGTGACGGACC |
| Sequence-based reagent | Mtpα_R | This paper | PCR primers | CTGCAGCAGTCTGATGGCTT |
| Sequence-based reagent | scully_F | This paper | PCR primers | GATCAAGAACGCCGTTTCCC |
| Sequence-based reagent | scully_R | This paper | PCR primers | CAGATCGGCCAGGATCACG |
| Sequence-based reagent | Mtpβ_F | This paper | PCR primers | CAGGCACTCGCTTTTGTCAT |
| Sequence-based reagent | Mtpβ_R | This paper | PCR primers | CCTGGCAATGTTGGAGGTCT |
| Sequence-based reagent | yip2_F | This paper | PCR primers | TCTGCCGCAACCAAAGGTAT |
| Sequence-based reagent | yip2_R | This paper | PCR primers | TTAAGACCGGCAGCATCCAG |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | Photoshop CC | Adobe | RRID:SCR_014199 | |
| Software, algorithm | Imaris | Bitplane | RRID:SCR_007370 | |
| Commercial assay or kit | Click-iT EdU plus (DNA replication kit) | Invitrogen | C10639 | |
| Commercial assay or kit | ATP bioluminescence kit HSII | Sigma | 11699709001 | |
| Commercial assay or kit | Histone extraction kit | Abcam | ab113476 | |
| Commercial assay or kit | RNAeasy Mini Kit | Qiagen | 74104 |