Video 1. Calcium imaging of femur muscles.

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The video (1) illustrates the arrangement of musculature controlling the fly’s tibia; (2) schematizes the position of a restrained fly relative to the force probe fiber, together with wide-field calcium imaging from muscles in the leg and body; and then (3) shows simultaneous videos of the fly pulling on the force with muscle calcium signals in the femur, together with the movement of the probe, an EMG recording from the fast neuron, and the time course of cluster ΔF/F. Videos are slowed 3X.