Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | ‘w[*]; P{w[+mC]=Mhc-GAL4.K}2’ | Bloomington Drosophila Stock Center | RRID:BDSC_55133 | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=20XUAS-IVS-mCD8::GFP}attP2’ | Bloomington Drosophila Stock Center | RRID:BDSC_32194 | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=GMR22A08-GAL4}attP2’ | Bloomington Drosophila Stock Center | RRID:BDSC_47902 | |
| Genetic reagent (D. melanogaster) | ‘P{JFRC7-20XUAS-IVS-mCD8::GFP} attp40’ | Other | FBrf0212432 | Barret Pfeiffer, Janelia Farm, HHMI |
| Genetic reagent (D. melanogaster) | ‘MHC-LexA’ | Other | Richard Mann, Columbia University | |
| Genetic reagent (D. melanogaster) | ‘13XLexAop2-IVS-GCaMP6f-p10}su(Hw)attP5’ | Bloomington Drosophila Stock Center | RRID:BDSC_44277 | |
| Genetic reagent (D. melanogaster) | ‘Berlin-K’ | Bloomington Drosophila Stock Center | RRID:BDSC_8522 | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=GMR81A07-GAL4}attP2’ | Bloomington Drosophila Stock Center | RRID:BDSC_40100 | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=GMR35 C09-GAL4}attP2’ | Bloomington Drosophila Stock Center | RRID:BDSC_49901 | |
| Genetic reagent (D. melanogaster) | ‘10XUAS-syn21-Chrimson88-tDT3.1 (attP18)’ | DOI:10.1016/j.neuron.2017.03.010 | Michael Reiser, Janelia Farm, HHMI | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=20 XUAS-IVS-CsChrimson.mVenus}attP2’ | Bloomington Drosophila Stock Center | RRID:BDSC_55136 | |
| Genetic reagent (D. melanogaster) | ‘iav-LexA’ | Bloomington Drosophila Stock Center | RRID:BDSC_52246 | |
| Genetic reagent (D. melanogaster) | ‘13XLexAop2-IVS-Syn-21-Chrimson::tdTomato (attP2)’ | DOI: 10.1038/nmeth.2836 | Barret Pfeiffer, Janelia Farm, HHMI | |
| Genetic reagent (D. melanogaster) | ‘P{y[+t7.7] w[+mC]=BDP-GAL4}attP2’ | DOI:10.7554/eLife.08758.027 | Andrew Seeds, Steffi Hampel, UNIVERSITY OF PUERTO RICO | |
| Genetic reagent (D. melanogaster) | ‘P{w[+mW.hs]=GawB}VGlut[OK371]’ | Bloomington Drosophila Stock Center | RRID:BDSC_26160 | |
| Antibody | nc82 (mouse monoclonal) | Developmental Studies Hybridoma Bank, | RRID:AB_2314866 | 1:50 |
| Chemical compound, drug | MLA | Tocris | TOCRIS_1029 | ‘1 μM methyllycaconitine citrate’ |
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | DeepLabCut | DOI:10.1038/s41593-018-0209-y | Mathis Lab, Rowland Institute, Harvard University https://github.com/AlexEMG/DeepLabCut | |
| Software, algorithm | FIJI | PMID:22743772 | RRID:SCR_002285 | |
| Software, algorithm | Fictrac | DOI:10.1016/j.jneumeth.2014.01.010 | ||
| Other | Force probe fiber | Proform CS2.5 AS | This paper | “PBT fiber from a synthetic paint brush, cut to have a spring constant = 0.22 N/m. Find at any Proform retailer, e.g. Amazon.com’ |
| Other | Green CST DPSS laser, | Besram Technology, Inc | ‘532 nm’ | |
| Other | Phalloidin | ThermoFisherScientific | FISHER: A22284 | ‘633 nm, 1 unit per leg’ |
| Other | streptavidin-Alexa Fluor | ThermoFisherScientific | FISHER: S11226 | ‘568 nm’ |