Table 2.
Primer names, target genes, oligonucleotide sequences, and the product size used in PCR
| Pathotype | Primer name (target gene) | Oligonucleotide sequences (5′–3′) | Product size (bp) | References |
|---|---|---|---|---|
| For molecular identification of E. coli | ||||
| E16S (16S rRNA) |
F: CCCCCTGGACGAAGACTGAC R: ACCGCTGGCAACAAAGGATA |
401 | Wang et al. (2002) | |
| For detection of virulence factors encoding genes | ||||
| EPEC | EAE (eae) |
F: TCAATGCAGTTCCGTTATCAGTT R: GTAAAGTCCGTTACCCCAACCTG |
482 | Vidal et al. (2005) |
| STEC | Stx1 (stx1) |
F: CGATGTTACGGTTTGTTACTGTGACAGC R: AATGCCACGCTTCCCAGAATTG |
244 | Müller et al. (2007) |
| Stx2 (stx2) |
F: CCATGACAACGGACAGCAGTT R: CCTGTCAACTGAGCAGCACTTTG |
779 | Gannon et al. (1992); Kamel et al. (2015) | |
| HlyA (hlyA) |
F: AGCTGCAAGTGCGGGTCTG R: TACGGGTTATGCCTGCAAGTTCAC |
569 | Wang et al. (2002) | |
| ETEC | LT (lt) |
F: GGCGACAGATTATACCGTGC R: CGGTCTCTATATTCCCTGTT |
450 | Stacy-Phipps et al. (1995) |
| ST (st) |
F: ATTTTTMTTTCTGTATTRTCTT R: CACCCGGTACARGCAGGATT |
190 | ||
| Fimbrial F5 (k99) |
F: TATTATCTTAGGTGGTATGG R: GGTATCCTTTAGCAGCAGTATTTC |
314 | Franck et al. (1998) | |
| EAEC | EAST1 (astA) |
F: TGCCATCAACACAGTATATCCG R: ACGGCTTTGTAGTCCTTCCAT |
102 | Müller et al. (2007) |