. Author manuscript; available in PMC: 2021 Jul 7.

Published in final edited form as: Structure. 2020 Apr 30;28(7):760–775.e8. doi: 10.1016/j.str.2020.04.009

Table 2.

Protein α-carbon rmsd values

	eOnuTherm	E178D	Intermediate #1	Intermediate #2	Final
		E178D	+T203S,D236E	+I86S, S188K, S190T, Q197K	+N32Y, N33S, K34Q, S35T, S36R, S40L
eOnuTherm	-	0.57 Å	0.52 Å / (−4°)^*	0.47 Å / (−5°)^*	0.43 Å / (−8°)^*
E178D		---	0.20 Å	0.29 Å	0.40 Å
Intermediate #1			---	0.23 Å	0.38 Å
Intermediate #2				---	0.28 Å
Final					---

Values in parentheses are the decrease in denaturation midpoint temperatures (Tm values) relative to the ‘eOnuTherm’ scaffold.