KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Biotin anti-HA.11 Epitope Tag, Clone 16B12 Lot # B293295 |
BioLegend | Cat# 901505 RRID: AB_2565023 |
| anti-C-Myc Antibody (Chicken) - FITC Conjugated Lot #48 | ICL | Cat# CMYC-45F |
| PE Streptavidin (SAV-PE) Lot #9049827 | BD Biosciences | Cat# BDB554061 |
| Bacterial and Virus Strains | ||
| BL21-Codon Plus (DE3)-RIL Escherichia coli competent cells | Agilent | Cat# 230245 |
| Novagen BL21(DE3) pLysS Escherichia coli competent cells | EMD Millipore | Cat# 69451 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bacto™ Yeast Extract | ThermoFisher | Cat# 212750 |
| Gibco™ Bacto™ Peptone | Fisher Scientific | Cat# DF0118-17-0 |
| alpha-D(+)-Glucose, 99+%, anhydrous, Acros Organics™ | Fisher Scientific | Cat# AC170080010 |
| Adenine hemisulfate salt | Sigma-Aldrich | Cat# A3159 |
| Gibco™ Penicillin-Streptomycin (10,000 U/mL) | ThermoFisher | Cat# 15140122 |
| Kanamycin Sulfate (White Powder), Fisher BioReagents | Fisher Scientific | Cat# BP906-5 |
| MP Biomedicals™ D-(+)- Raffinose pentahydrate (powder) | Fisher Scientific | Cat# MP114061522 |
| D(+)-Galactose, 99+%, Acros Organics™ | Fisher Scientific | Cat#AC150615000 |
| YNB + Nitrogen Powder | Sunrise Science Products | Cat# 1501-500 |
| Casamino acids | Fisher Scientific | Cat# J851-1KG |
| MES (Fine White Crystals), Fisher BioReagents | Fisher Scientific | Cat# BP300-100 |
| Thermo Scientific Bacteriological Agar, Ultrapure | Fisher Scientific | Cat# AAJ10906P2 |
| Deoxyribonucleic acid sodium salt from salmon testes | Sigma-Aldrich | Cat# D1626-1G |
| 50% w/v Polyethylene glycol 3,350 Monodisperse | Hampton Research | Cat# HR2-527 |
| Platinum™ Taq DNA Polymerase High Fidelity | Invitrogen | Cat#11304029 |
| Exonuclease I (E.coli) | New England Biolabs | Cat# M0293S |
| Ficoll PM 400 | Sigma-Aldrich | Cat# F4375 |
| AccuPrime™ Pfx DNA Polymerase | Invitrogen | Cat# 12344024 |
| Luria Broth (LB) 1 Liter buffered capsules | RPI | Cat# L24045 |
| Ampicillin sodium salt (crystalline powder) | Fisher Scientific | Cat# BP1760 |
| Isopropyl β-D-1-thiogalactopyranoside (IPTG) | Teknova | Cat# I3326 |
| Phenylmethylsulfonyl fluoride (PMSF) | Sigma-Aldrich | Cat# P7626 |
| Benzonase Nuclease | Sigma-Aldrich | Cat# E1014-25KU |
| Critical Commercial Assays | ||
| Deposited Data | ||
| I-OnuI structure | Takeuchi et al., 2011 | PDB: 3QQY |
| I-OnuI-e-Therm (eOnuTherm) structure | This paper | PDB: 6UVW |
| I-OnuI-e-ThermE178D (eOnuTherm-E178D) structure | This paper | PDB: 6UWG |
| I-OnuI-e-Therm-bCtxA (eOnuTherm-bCtxA) structure | This paper | PDB: 6UW0 |
| I-OnuI-e-Therm-hChr11v1 (Intermediate #1) structure | This paper | PDB: 6UWH |
| I-OnuI-e-Therm-hChr11v2 (Intermediate #2) structure | This paper | PDB: 6UWJ |
| I-OnuI-e-Therm-hChr11v3 (eOnuTherm-hChr11) structure | This paper | PDB: 6UWK |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Saccharomyces cerevisiae (Strain: EBY100) | ATCC | Cat# MYA-4941 |
| Oligonucleotides | ||
| See Table S2 for oligonucleotides used in this study | Integrated DNA Technologies (IDT) | N/A |
| Recombinant DNA | ||
| pETCON yeast surface display vector | Baxter, S.K. et al., 2014 | Addgene Cat# 41522 |
| Novagen pET21d bacterial protein expression vector | EMD Millipore | Cat# 69743-3 |
| Software and Algorithms | ||
| PROSS: the Protein Repair One-Stop Shop | Goldenzweig, A. et al., 2016 | https://pross.weizmann.ac.il/step/pross-terms/ |
| FlowJo™ Software for Mac. Version 9.9.6. | Ashland, OR: Becton, Dickinson and Company; 2019. | https://www.flowjo.com |
| Spectra Manager II Version 2.10.1.1 | JASCO | https://www.jasco.de/Spectroscopy/Software |
| HKL-2000 v715 for Mac | HKL Research, Inc | https://hkl-xray.com/download-instructions-hkl-2000 |
| Phenix Software Suite Version 1.17-3644 | Liebschner, D. et al., 2019 | https://www.phenix-online.org |
| COOT Crystallographic Object-Oriented Toolkit Version 0.8.9.2 |
Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PyMOL Molecular Graphics System Version 2.3.0 | Schrödinger, LLC | https://pymol.org |
| Other | ||
| illustra™ Sephadex™ G-100 DNA grade | GE Healthcare | Cat# 17057402 |
| HiTrap® Heparin High Performance column | GE Healthcare | Cat# 17-0407-03 |
| HiLoad® 16/600 Superdex® 75 prep grade column | GE Healthcare | Cat# 28-9893-33 |