Figure 5.

LIM kinase (LIMK) inhibition prevents arteriolar inward remodeling in a mouse model of angiotensin-II (AngII) infusion-dependent hypertension. Mice were infused with AngII (100 or 1000ng/kg/min), while one of their ears was injected subcutaneously with vehicle control and the contralateral with 20μL LIMKi (100μM). A, Carotid-catheterization-acquired mean arterial pressure in mice implanted with minipumps delivering AngII at 100 or 1000ng/kg/min. B, Changes in auricular subcutaneous arteriolar diameter expressed as percent remodeling after 14 days of AngII infusion in the ears treated with vehicle or LIMKi. C, Representative multiphoton intravital images of arterioles (green) within an ear treated with vehicle on days 0 and 14 of AngII (1000ng/kg/min) infusion. Data are expressed as means ± SEM. *P<0.05 vs. AngII (100ng/kg/min). ^$P<0.05 vs. AngII (1000ng/kg/min) + vehicle, n=4 / treatment group.