Table 1.

Comparison of IPA “Leptin Signaling in Obesity” gene expression signature enrichment between cells assigned to each cluster, from chow- vs HFD-fed animals.

Cluster	Cell type	Genes within cluster that match “Leptin Signaling in Obesity” gene expression signature & exhibit significant changes in expression with HFD	Z-score	P-value
0	Gm42418/AY036118	Pomc	(none)	0.197
1	Gm42418/AY036118	Plcb4, Pik3c2a, Map2k2, Akt3	(none)	0.288
2	Slc17a6/Bdnf	Pik3c2a, Fgfr1, Pomc	(none)	1.000
3	Sst/Dlk1	Npy, Agrp	(none)	0.048
4	Gda/Rasgrf2	Pomc	(none)	0.238
5	Pomc/Cartpt	Agrp, Pomc	(none)	0.201
6	Gad2/Zic1	(none)	(none)	(none)
7	Astrocytes 1	Fgfr3, Prkacb, Plce1, Pomc	(none)	0.044
8	Foxb1/Rprm	Npy, Plcb4, Mapk1, Fgfr1, Plcb1, Pomc, Stat3, Adcy8	−0.447	0.031
9	unkSlc18a/Hdc	(none)	(none)	(none)
10	Oligodendrocytes 1	Pik3r3, Gab1, Fgfr1, Fgfr2, Plcb1, Agrp, Pomc	0.000	0.003
11	Oligodendrocytes 2	Pik3ca	(none)	0.413
12	Endothelial Cells	Pde3a, Pomc	(none)	0.252
13	Astrocytes 2	Mapk3, Pomc	(none)	1.000
14	Tanycytes	(none)	(none)	(none)
15	Agrp/Npy	Prkar2b, Ptpn11, Fgfr1, Plcb1, Agrp, Pomc, Stat3, Map2k1	−1.633	0.009
16	Microglia	Prkar2b, Pik3r2	(none)	1.000
17	Ghrh/Th	Plcb4, Prkar2b, Adcy1	(none)	0.188
18	Oligodendrocytes 3	Gab2, Akt1, Prkar2b, Map2k2, Pik3r1, Pomc, Jak2, Pik3r2	−1.633	0.122
19	Astrocytes 3	Prkar2a	(none)	1.000
20	Tac1/Foxp2	Lepr	(none)	0.316
21	Rgs16	Mapk1	(none)	0.324
22	Cpne9/Foxb1	Lepr, Plcg1, Pik3r2	(none)	0.154

Additional information included in Supplemental File 2.