Key Resources Table
| Reagent or resource | Source | Identifiers |
|---|---|---|
| Bacterial and Virus Strains | ||
| BL21 dE3 Chemically Competent E. coli | Novagen | 69450 |
| BL21 dE3 AI Chemically Competent E. coli | ThermoFisher Scientific | C607003 |
| Origami 2 dE3 Chemically Competent E. coli | Novagen | 71345 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| cOmplete™, EDTA-free Protease Inhibitor Cocktail | Sigma-Alrich | 4693132001 |
| Adenosine 5’-triphosphate disodium salt hydrate | Sigma-Aldrich | A2383 |
| BS3 (bis(sulfosuccinimidyl)suberate) | ThermoFisher Scientific | A39266 |
| Mouse anti-LRH-1 primary | R&D Systems | PP-H2325-00 |
| Goat anti-mouse HRP conjugated secondary | Promega | W4021 |
| β-actin primary | Millipore Sigma | A3854 |
| PGC1α peptide (740EEPSLLKKLLLAPA753) | New England Peptides | N/A |
| N-terminally FAM-labeled PGC1α peptide (740EEPSLLKKLLLAPA753) | New England Peptides | N/A |
| Gene | ||
| NR5A2 (LRH-1), Homo sapiens | UniProt database | O00482 |
| Deposited Data | ||
| LRH-1FL Integrated Model | This study | PDBDev_00000035 |
| LRH-1 DBD:CYP7A1 Promoter Complex | Solomon et al, 2005 | PDB: 2A66 |
| LRH-1 LBD: NCoA2 Peptide Complex | Krylova, et al 2005 | PDB: 1YOK |
| LRH-1 LBD: RJW100: PGC1α Peptide Complex | Mays et al, 2017 | PDB: 5UNJ |
| LRH-1 LBD: DLPC: NCoA2 Peptide Complex | Musille et al, 2012 | PDB: 4DOS |
| mSF-1 DBD: Inhibinα Promoter Complex | Little et al, 2006 | PDB: 2FF0 |
| Experimental Models: Cell Lines | ||
| HEK 293T, Human embryonic kidney | ATCC | CRL-3216 |
| Recombinant DNA | ||
| pRSF2-LRH-1 WT | Suzawa, et al., 2015. | N/A |
| pRSF2-LRH-1 S148R | This study | N/A |
| pRSF2-LRH-1 R174D | This study | N/A |
| pRSF2-LRH-1 D314R | This study | N/A |
| pRSF2-LRH-1 R174D/D314R | This study | N/A |
| pRSF2-LRH-1 A324R | This study | N/A |
| pRSF2-LRH-1 A324TAG | This study | N/A |
| pRSF2-LRH-1 Q126C/Y528C | This study | N/A |
| pEVOL-pBpF | Chin et al., 2002 | Addgene 31190; A gift from Peter Schultz |
| pET-His-MBD-TEV- LRH-1 DBD (aa 79–187) | This study | N/A |
| pET-His- TEV- LRH-1 LBD WT (aa 266–541) | This study | N/A |
| pET-His- TEV- LRH-1 LBD D314R (aa 266–541) | This study | N/A |
| pET28-His-TEV- human β-catenin R1C WT | Addgene 17200; A gift from Randall Moon | |
| pET-His-TEV- human β-catenin R1C K306A / K345A / W383A | This study | N/A |
| pET-His- mouse Dax-1 WT (aa 205–472) | This study | N/A |
| pET-His- mouse Dax-1 L279A / L280A (aa 205472) | This study | N/A |
| HA-LRH1 WT, mCherry dual ORF | This study | N/A |
| HA-LRH1 S148R, mCherry dual ORF | This study | N/A |
| HA-LRH1 R174D, mCherry dual ORF | This study | N/A |
| HA-LRH1 R174E/D314E, mCherry dual ORF | This study | N/A |
| HA-LRH1 K179A/K182A/K183A, mCherry dual ORF | This study | N/A |
| HA-LRH1 D314R, mCherry dual ORF | This study | N/A |
| pcDNA3-S33Y human β-catenin | Kolligs et al., 1999 | Addgene 19286; A gift from Eric Fearon |
| pcDNA3 human DAX-1 | Genscript | O H u 2 8 2 9 5 |
| CYP17A1-pGL3-Basic | A gift from Holly Ingraham | |
| pRL-SV40 | Promega | E2231 |
| pRL-TK | Promega | E2241 |
| Oligonucleotides | ||
| CYP7A1 Forward Promoter: GTTCAAGGCCAG | IDT | N/A |
| FAM-labeled CYP7A1 Forward Promoter: GTTCAAGGCCAG | IDT | N/A |
| CYP7A1 Reverse Promoter: CTGGCCTTGAAC | IDT | N/A |
| Software and Algorithms | ||
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| pLink 2.0 | Chen et al, 2019 | http://pfind.ict.ac.cn/software/pLink/ |
| Crossfinder | Mueller-Planitz | https://www.molekularbiologie.abi.med.uni-muenchen.de/ueber_uns/mueller_planitz/index.html |
| Skyline | MacLean et al, 2010 | https://skyline.ms/project/home/software/Skyline/begin.view |
| Rosetta 3.10 | Leaver Fay et al., 2011 | https://www.rosettacommons.org/software |
| ATSAS 2.8.4 | Franke D et al., 2017 | https://www.embl-hamburg.de/biosaxs/software.html |
| REMO | Li Y et al., 2009 | https://zhanglab.ccmb.med.umich.edu/REMO/ |
| BCL::Conf | Kothiwale S et al., 2015 | http://www.meilerlab.org/index.php/ |
| AMBER16 | Case DA et al., 2016 | http://ambermd.org |
| PyMOL | The PyMOL Molecular Graphics System, Version 2.0 Schrodinger, LLC | https://pymol.org/ |
| VMD | Humphrey W et al., 1996 | https://www.ks.uiuc.edu/Research/vmd/ |
| CPPTRAJ | Roe DR & Cheatham TE 3rd, 2013 | http://ambermd.org/AmberTools.php |
| CARMA | Glykos NM, 2006 | https://utopia.duth.gr/~glykos/Carma.html |
| NetworkView plugin for VMD | Eargle J et al., 2012 | https://www.ks.uiuc.edu/Research/vmd/plugins/networkview/ |
| ClustalOmega | Madeira et al, 2019 | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| PSIPRED | Jones et al, 1999 | http://bioinf.cs.ucl.ac.uk/psipred/ |
| RosettaRemodel | Huang, et al 2011 | https://www.rosettacommons.org/software |
| Other | ||
| TALON metal affinity resin | Takara | 635503 |
| Superdex 200 Increase HiScale 26/40 | GE Healthcare | 29321906 |
| Superdex 75 Increase 10/300 GL | GE Healthcare | 29148721 |
| Protein Assay Dye Reagent Concentrate | Bio-Rad | 5000006 |
| Dual Luiferase® Reporter Assay System | Promega | E1980 |