Table 2.
Details of the automatic assessment of multiple P-Rex1 cryo-EM data sets.
| 18sep06b | 18sep28b | 18jan09b | 18jan10d | 18jul14a | 18jan18c | |
|---|---|---|---|---|---|---|
| P-Rex1 concentration (μM) | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 |
| Additive (μM) | - | - | DDM (80) | Lubrol (40) | DDM (80) | DDM (80) |
| Gβγ concentration (μM) | - | - | - | - | 60 | 6.0 |
| Grid type | Quantifoil 1.2/1.3 | Lacey carbon | Quantifoil 1.2/1.3 | Quantifoil 1.2/1.3 | Quantifoil 1.2/1.3 | Quantifoil 1.2/1.3 |
| Microscope | Titan Krios | Talos Arctica | Talos Arctica | Talos Arctica | Titan Krios | Titan Krios |
| Original Pixel Size (Å) | 1 | 0.91 | 0.91 | 0.91 | 1 | 1 |
| Number of total micrographs | 1,716 | 1,491 | 1,206 | 1,110 | 1,352 | 5,011 |
| Number of good micrographs # | 986 | 445 | 841 | 790 | 1,217 | 4,157 |
| Estimated diameter (Å) | 144 | 144 | 135 | 132 | 138 | 151 |
| Number of total picked particles | 178,483 | 37,946 | 177,086 | 205,682 | 424,213 | 921,403 |
| Number of good particles | 94,514 | 9,535 | 114,630 | 141,982 | 145,941 | 492,883 |
| Pixel size for 2D classification (Å) | 4 | 3.59 | 3.64 | 3.67 | 3.94 | 3.97 |
| Best diameter for 2D classification (Å) | 129 | 216 | 108 | 105 | 110 | 120 |
| Workflow CPU core hours (Intel Xeon E5–2660 v3) | 3945 | 4166 | 13712 | 7516 | 4347 | 1251 |
| Workflow GPU hours (NVIDIA GTX 1080 Ti) | ~2 | ~2 | ~2 | ~2 | ~2 | ~2 |