. Author manuscript; available in PMC: 2021 Jul 7.

Published in final edited form as: Structure. 2020 Apr 14;28(7):858–869.e3. doi: 10.1016/j.str.2020.03.008

Table 3.

Overview of cryo-EM structures.

	T20S Proteosome (EMPIAR-10025)	HA Trimer (EMPIAR-10175)	Aldolase
Microscope	Titan Krios	Titan Krios	Talos Arctica
Detector	Gatan K2	Gatan K2	Gatan K2
Voltage (kV)	300	300	200
Electron exposure (e⁻/Å²)	53	73.24	44.13
Defocus range (μm)	0.9 – 2.4	1.0–2.1	0.8 – 2.0
Original pixel size (Å)	0.66	0.85	0.91
Symmetry imposed	D7	C3	D2
Initial particle images (no.)	52,153	167,788	536,520
Final pixel size (Å)	0.88	1.275	1.22
Final particle images (no.)	45,066	150,684	425,087
FSC threshold	0.143	0.143	0.143
Map resolution (A)	3.0	3.2	3.2
B-factor (Å ²)	−94	−151	−110
Workflow CPU core hours (Intel Xeon E5–2660 v3)	1600	2353	8293
Workflow GPU hours (NVIDIA GTX 1080 Ti)	~2	~2	~2