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. 2020 Jul 9;10:11393. doi: 10.1038/s41598-020-68257-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dCas9-KRAB alters histone modifications at the PTEN transcriptional regulatory region. (A,B) dCas9-KRAB was stably expressed in the HEK 293T cell line with gRNA D targeting the PTEN 5′ UTR, or with no gRNA. ChIP with H3K9me3 (A) and H3K9ac (B) antibodies was performed, followed by qPCR with primers in PTEN and GAPDH regulatory regions. Results are expressed as the percentage of immunoprecipitated DNA relative to input chromatin, *p < 0.05, **p < 0.01 (Student’s t test), n = 3, error bars show SEM.