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. 2020 Jul 9;10:11329. doi: 10.1038/s41598-020-68187-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PC-3 and DU145 generate morphologically heterogeneous colonies using a colony formation assay. (a) Representative images of holoclones 5 days and 7 days following plating. Also shown are the inner cellular composition of holoclones and the border composition. (b) Representative images of meroclones and paraclones. (Images are representative of three independent experiments).