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. 2020 Jul 9;10:11329. doi: 10.1038/s41598-020-68187-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative E-Cadherin, Vimentin and CD34 staining in parent and holoclone-derived tumour sections. (a) E-cadherin expression in PC-3 and DU145 parental and holoclone tumours. E-cadherin expression was observed as diminished in the holoclone-derived tumour sections. Arrows depict areas of interest. (b) Vimentin expression in PC-3 and DU145 parental and holoclone tumours. Little difference in vimentin expression was observed between parent and holoclone samples. Arrows depict areas of interest. (c) CD34 staining in PC-3 parental and holoclone-derived tumour. No detectable vessel staining was observed in either parental or holoclone tumour samples. Representative images from three tumour samples.