Table 2.
IHC antibodies.
| Incubation time (min) | Optimal dilution | Company | Ref. code | |
|---|---|---|---|---|
| 1° antibody | ||||
| Anti-ERG Rabbit mAB | 32 | Neat | Roche | EPR3864 |
| Anti-Ki-67 Mouse mAB | 32 | 1:80 | Dako | IS62630-2 |
| Anti-CCND1 Rabbit mAB | 24 | Neat | Dako | IS08330-2 |
| Anti-Cytokeratin (Cam5.2) Rabbit mAB | 16 | Neat | Roche | 790-4555 |
| Anti-E-cadherin Mouse mAB | 32 | 1:50 | Invitrogen | 18-0223 |
| Anti-CD34 Mouse mAB | 32 | 1:50 | Dako | IR63261-2 |
| Anti-Vimentin Mouse mAB | 16 | Neat | Dako | IR63061-2 |
| 2° antibody | ||||
| OmniMap anti-Rabbit HRP | 20 | Neat | Roche | 760-4311 |
| Universal Secondary Antibody MultiLink | 20 | Neat | Dako | E045301-2 |