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. 2020 Jul 9;10:11316. doi: 10.1038/s41598-020-68172-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Her9 expression in the developing retina. Fluorescent in situ hybridization (FISH) showing her9 mRNA expression at 24 (A–A′), 48 (B–B′), and 72 (C–C′) hpf in the developing retina (expression in the lens at 72 hpf is auto-fluorescence). (D–D′) × 100 magnification of boxed area in (C–C′) showing her9 expression in the CMZ. her9 sense probes at 24, 48, and 72 hpf are shown in (A″, B″, C″, D″). Panels in (A″–D″) display her9 sense probe without the DAPI staining. L, lens CMZ, ciliary marginal zone; NR, neural retina; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 50 μm and 100 μm.