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. 2020 Jul 9;10:11316. doi: 10.1038/s41598-020-68172-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Her9 mutants display cone subtype-specific phenotypes. Immunohistochemistry using a green (A–B′), UV (C–D′), or blue (E–F′) cone opsin antibody on WT and her9 mutant retinal cryosections. (G–I) Cell counts of opsin expressing cells in her9 mutant retinas compared to their siblings (# of opsin+ cells/100 µm). Green Opsin: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p < .0004). UV Opsin: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p < .0046). Blue Opsin: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p = .1892). (J) qPCR analysis of the different cone opsins at 8 dpf (fold change relative to Ef1α). ONL, outer nuclear layer. Scale bar = 50 µm.