Fig. 1. CR9114 expression in vitro and in vivo.

To measure the CR9114 antibody level in vitro, different quantities of AdC68–CR9114 (10¹⁰, 10⁹, or 10⁸ virus particles (vp)) were used to infect the HEK293 cells in a 6-well plate. AdC68-gp (10¹⁰ vp) was used as the negative control, while the uninfected HEK293 cells were used as the blank controls. Supernatants were harvested at 24 and 48 h after infection. For the in vivo expression of the CR9114 antibody, mice were randomly divided into four groups (5 mice per group), and intranasally immunized with AdC68–CR9114 or AdC68–empty at 5 × 10¹⁰ vp/mouse in 30 μl of PBS, or intravenously administered AdC68–CR9114 or AdC68–empty at 5 × 10¹⁰ vp/mouse in 200 μl of PBS. All mice were subjected to bleeding every other day. a Western blot detection of the CR9114 antibody in the supernatant of infected HEK293 cells under nonreducing and reducing conditions. b The purified CR9114 antibody was boiled and subjected to SDS-PAGE with Coomassie Blue R-250 staining. c, d The concentrations of CR9114 in vitro and in vivo (five mice per group). Data in c, d are expressed as mean ± SD.