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. 2020 Jul 9;11:3421. doi: 10.1038/s41467-020-17293-3

Fig. 7. Human cDC2 upregulate OX40L after treatment with recombinant IFNγ or co-culture with autologous NK cells activated by IL-12/15.

Fig. 7

To investigate whether NK cell crosstalk regulated OX40L expression on human DCs, cDC2 and NK cells were isolated to high purity by FACS and cultured in vitro. a Representative flow cytometric plots showing the expression of OX40L and CD40 on cDC2 (CD14− CD19− CD3− HLA-DR+ CD1c+) after 40 h of culture in the indicated conditions. b Percentage of cDC2-expressing OX40L under the conditions indicated. c MFI of OX40L expression by cDC2 under the conditions indicated. Data were pooled from 3 independent experiments (n = 6 patient samples). Values on flow cytometric plots represent percentages; bars on scatter plots represent the median. Statistical significance was tested by using Kruskal–Wallis one-way ANOVA with post hoc Dunn’s test: *p ≤ 0.05, **p ≤ 0.01.