Table 1.

Efficiency in the establishment of the naturally conceived and vitrified-transferred progenies across three generations (F1, F2, F3).

Experimental groups	F1 generation				F2 generation				F3 generation
	Founding parities	Litter size	Live births	Adult males	F1 parities	Litter size	Live births	Adult males	F2 parities	Litter size	Live births	Adult males
Naturally conceived	14	5.5 ± 0.62	77	35	10	6.1 ± 0.76	61	13	10	6.1 ± 0.89	61	24
Vitrified-transferred	13	5.3 ± 0.64	69	30	10	5.6 ± 0.76	56	9	10	6.4 ± 0.89	64	24

In F1 generation, naturally-conceived animals were generated from 14 pregnant females following natural conception, without embryo manipulation. Instead, vitrified-transferred animals were generated from vitrified-warmed embryos transferred in 13 foster mothers. F2 and F3 generations were produced selecting randomly one mature female and male from each parity of the previous generation. To reduce the inbreeding, mating between animals with common grandparents was avoided. Thereby, 10 F1 and 10 F2 parities generated F2 and F3 population, respectively.