Table 2.
Ejaculates/sperm parameters and motility assessment of males from the vitrified-transferred progeny (VT) compared to those naturally conceived (NC).
| Generation | F1 | F2 | F3 | |||
|---|---|---|---|---|---|---|
| Experimental group | NC | VT | NC | VT | NC | VT |
| N | 76 | 173 | 72 | 62 | 120 | 135 |
| Semen parameters | ||||||
| VOL (mL) | 0.69 ± 0.050 | 0.89 ± 0.033* | 0.60 ± 0.033 | 0.80 ± 0.036* | 0.51 ± 0.0211 | 0.44 ± 0.0202 |
| CON (106) | 238.5 ± 15.69 | 201.7 ± 10.40 | 181.5 ± 8.45 | 172.5 ± 9.11 | 305.6 ± 17.56 | 307.5 ± 16.79 |
| TSE (106 spz) | 156.8 ± 15.70 | 179.1 ± 10.40 | 92.1 ± 6.92 | 137.0 ± 7.31* | 145.8 ± 8.15 | 126.5 ± 7.57 |
| MOT (%) | 71.9 ± 1.93 | 76.9 ± 1.28 | 90.0 ± 0.67 | 88.5 ± 0.65 | 52.1 ± 2.61 | 53.7 ± 2.46 |
| PRO (%) | 40.5 ± 1.73 | 43.0 ± 1.16 | 55.9 ± 1.30 | 59.8 ± 1.42 | 27.5 ± 1.74 | 29.5 ± 1.64 |
| VIA (%) | 74.5 ± 1.13 | 74.1 ± 0.77 | 82.6 ± 0.69 | 83.0 ± 0.75 | 73.1 ± 1.22 | 73.2 ± 1.15 |
| NAR (%) | 90.3 ± 0.83 | 90.3 ± 0.55 | 88.4 ± 0.55 | 88.2 ± 0.61 | 92.9 ± 0.56 | 92.3 ± 0.53 |
| ABN (%) | 19.6 ± 0.98 | 20.7 ± 0.64 | 18.4 ± 0.60 | 14.9 ± 0.66* | 22.8 ± 0.97 | 23.1 ± 0.92 |
| Motion parameters | ||||||
| VCL (μm s−1) | 86.6 ± 2.34 | 99.8 ± 1.55* | 99.8 ± 1.44 | 96.2 ± 1.53 | 104.5 ± 2.17 | 96.4 ± 2.05* |
| VSL (μm s−1) | 34.0 ± 1.47 | 40.4 ± 0.98* | 55.9 ± 1.18 | 55.0 ± 1.28 | 38.8 ± 1.22 | 38.3 ± 1.15 |
| VAP (μm s−1) | 50.0 ± 1.87 | 59.9 ± 1.23* | 74.1 ± 1.31 | 72.9 ± 1.42 | 58.3 ± 1.25 | 55.8 ± 1.40 |
| LIN (%) | 40.3 ± 2.15 | 42.0 ± 1.42 | 58.7 ± 0.94 | 57.0 ± 0.98 | 37.9 ± 1.03 | 39.9 ± 0.97 |
| STR (%) | 68.9 ± 1.07 | 67.2 ± 0.71 | 77.1 ± 0.44 | 75.4 ± 0.46 | 66.1 ± 0.94 | 67.6 ± 0.88 |
| WOB (%) | 57.2 ± 1.32 | 59.3 ± 0.87 | 74.9 ± 0.99 | 76.0 ± 1.05 | 56.3 ± 0.95 | 58.1 ± 0.89 |
| ALH (μm) | 2.7 ± 0.55 | 2.8 ± 0.37 | 2.7 ± 0.42 | 2.7 ± 0.44 | 3.2 ± 0.06 | 3.0 ± 0.06 |
| BCF (Hz) | 10.8 ± 0.18 | 11.2 ± 0.12 | 9.9 ± 0.20 | 9.8 ± 0.22 | 12.2 ± 0.20 | 11.8 ± 0.19 |
Data are expressed as mean ± standard error of means.
n number of ejaculates, VOL ejaculate volume, CON spermatic concentration, TSE total sperm per ejaculate, spz spermatozoa, MOT percentage of sperm motility, PRO percentage of progressive motility, VIA percentage of viable sperm, NAR percentage of normal apical ridge, ABN percentage of abnormal forms, VCL curvilinear velocity, VSL straight-line velocity, VAP average path velocity, LIN linearity coefficient (VSL/VCL × 100), STR straightness coefficient, WOB wobble coefficient (VSL/VAP × 100), ALH amplitude of lateral head displacement, BCF beat cross-frequency.
*Asterisks denote relevant differences between VT traits and their NC counterparts (Bayesian inference: |DNC-VT|> R and P0 > 0.8; Supplementary Table S3).