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. 2020 Jul 9;10:11349. doi: 10.1038/s41598-020-68269-8

Figure 1.

Figure 1

The effect of TRPV4 deletion on gap closure of esophageal keratinocytes. (a) Representative images of cultured WT and TRPV4-KO keratinocytes stained with calcein. (b) The percentage of covered gap area was significantly larger 48 and 72 h after insert removal for cultured TRPV4-KO keratinocytes compared to WT. Data are presented as means ± SEM (n = 18–24). **p < 0.01 compared with WT, ‡‡p < 0.01 compared with WT 48 h, §§p < 0.01 compared with TRPV4-KO 48 h; as determined by t-test. (c) Representative images of cultured WT and TRPV4-KO keratinocytes transfected with mouse TRPV4 cDNA and stained with calcein. (d) Rescue transfection of TRPV4-KO esophageal keratinocytes with mouse TRPV4 cDNA significantly delayed gap closure. WT keratinocytes transfected with TRPV4 also exhibited significantly delayed gap closure. Data are presented as means ± SEM (n = 6–9). *p < 0.05; **p < 0.01 compared with WT, ‡‡p < 0.01 compared with mock-transfected WT, §§p < 0.01 compared with mock-transfected TRPV4-KO; as determined by t-test. White dotted lines indicate the edges of the cell-free gap at 0 h post-insert removal. Scale bars represent 200 μm.