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. 2020 Jul 9;10:11349. doi: 10.1038/s41598-020-68269-8

Figure 3.

Figure 3

Cyclic tensile strains delay gap closure of esophageal keratinocytes. (a) Representative images of cultured WT and TRPV4-KO keratinocytes exposed to 20% stretch at a frequency of 0.33 Hz for 72 h before calcein staining. The stretch was applied parallel or perpendicular to the gap alignment. White dotted lines indicate the edges of cell-free gap at 0 h post-insert removal. Scale bar represents 200 μm. (b) The percentage of covered gap area in WT and TRPV4-KO cultures following parallel or perpendicular stretch for 72 h. (c) Percent inhibition of gap closure induced by parallel or perpendicular stretch in WT and TRPV4-KO keratinocyte cultures. Data are presented as means ± SEM (n = 10–12). *p < 0.05; **p < 0.01 compared with WT, ‡‡p < 0.01 compared with WT control, §§p < 0.05; §§p < 0.01 compared with TRPV4-KO control; as determined by t-test.