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. 2020 Jul 9;11:3422. doi: 10.1038/s41467-020-17232-2

Fig. 7. INTS10–13–14 are required for termination after pausing.

Fig. 7

a Scheme of the RNAPII pause-termination reporter used in b. Reduced RNAPII pausing and termination at the HIV-1 TAR element results in elongation and increased RLuc levels. b Luciferase assay using the TAR pause-termination reporter. Cells depleted of individual INT subunits were transfected with the TAR reporter and FLuc transfection control plasmid. Statistical significance was determined for each knockdown condition relative to control. Bars show mean values of biological triplicates together with the individual replicates as dots. Error bars represent standard deviations. Statistical significance was determined by unpaired, two-tailed t-test: not significant (ns) p ≥ 0.05; significant *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Western blots in Fig. 6a. Source data are provided as a Source Data file. c Summary model of INT modules and their respective protein interaction networks. INTS10–INTS13–INTS14 brings cleavage module and target RNA into proximity.