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. 2020 Jul 9;10:11262. doi: 10.1038/s41598-020-67526-0

Figure 3.

Figure 3

mChABC increases Schwann cell migration and adhesion on astrocytes through CS-GAG removal. (a–f) Migration of Schwann cells (red; vibrant DiI) on astrocytes showing (a) control, (b) LV-fGFP, (c) bChABC treated, and (d) LV-mChABC populations. Scale bar = 100 μm; arrows indicate direction of migration; dashed line, the coverslip edge. Quantification of (e) cells migrating (N = 5, two-way ANOVA: cell type F(3,150) = 133.78, p < 0.0001, distance F(5,150) = 177.42, p < 0.0001) and (f) maximum distance (N = 5, one-way ANOVA: F(3,25) = 138.3, p = 0.0203). Cells were migrated on astrocyte matrix showing g) cells migrating (N = 5, two-way ANOVA: cell type F(3,48) = 311.15, p < 0.0001, distance F(5,48) = 897.39, p < 0.0001) and (h) maximum distance (N = 5, one-way ANOVA: F(3,8) = 134.4, p < 0.0001). (i–k) mChABC increases cellular adhesion on astrocytes. Showing (i) control, and (j) LV-mChABC populations stained with p75 (red) with (k) quantification (N = 20, one-way ANOVA: F(3,12) = 67.75, p < 0.0001). Scale bar = 100 μm. (l–m) anti-laminin, (o–q) anti-aggrecan (Cat 301), and (r–t) anti-CS-56 staining of astrocyte monolayer treated with medium from control, bChABC treated, LV-fGFP, and LV-mChABC populations. Scale bar = 40 μm. Quantified in (k) for laminin (N = 20, one-way ANOVA: F(3,76) = 1.873, p = 1.412); (n) aggrecan (N = 20, one-way ANOVA: F(3,76) = 614.6, p < 0.0001) and (t) CS-56 (N = 20, one-way ANOVA: F(3,76) = 708.6, p < 0.0001).