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. 2020 Jul 9;10:11262. doi: 10.1038/s41598-020-67526-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mChABC secretion aids neurite outgrowth over the Schwann cell-astrocyte boundary. (a–b) Images show Schwann cells (anti-p75; red) and DRG neurites (anti-βIII-tubulin; green) for (a) control, and (b) mChABC transduced cells. mChABC increases (c) the length of DRG neurites growing on Schwann cells (N = 3, one-way ANOVA: F(3,172) = 62.25, p < 0.0001) but (d) not the number of neurites (N = 3, one-way ANOVA: F(3,172) = 2.085, p = 0.1039). (e–g) Growth of neurites from (e) control, and (f) mChABC transduced Schwann cells (anti-p75; red) to astrocytes (anti-GFAP; green) or (h–j) astrocytes to Schwann cells with DRG neurites (anti-βIII-tubulin; blue). (g) mChABC increases the number of neurites crossing from Schwann cells to astrocytes (N = 3, one-way ANOVA: F(3,7) = 4.741, p = 0.0413). However, it does not increase the number that remain on the glial cell (N = 3, one-way ANOVA: F(3,7) = 2.07, p = 0.1928). (j) mChABC increases the number of neurites crossing from Schwann cells to astrocytes (N = 3, one-way ANOVA: F(3,7) = 0.8132, p = 0.05259). However, it does not increase the number that remain on the glial cell (N = 3, one-way ANOVA: F(3,7) = 3.804, p = 0.1148). For all panels, scale bar = 100 μm.