Table 1.
List of primary antibodies. (GFAP) Glial fibrillary acidic protein, (GFP) green fluorescent protein, (pFAK) phosphorylated FAK, (MBP) myelin basic protein, (ICC) immunocytochemistry, and (WB) western blot.
| Antibody | Clonality | Isotype | Host | Concentration | Company |
|---|---|---|---|---|---|
| 1B5 | IgG | Monoclonal | Mouse | 1:500 (ICC) 1:1,000 (WB) | Seikagaku |
| CS-56 | IgM | Monoclonal | Mouse | 1:300 (ICC) 1:1,000 (WB) | Sigma |
| Laminin | IgG | Polyclonal | Rabbit | 1:300 (ICC) | Sigma |
| βIII-tubulin | IgG | Polyclonal | Rabbit | 1:2000 (ICC) | Covance |
| βIII-tubulin | IgG | Monoclonal | Mouse | 1:200 | ThermoFisher Scientific |
| pFAK (try397) | IgG | Polyclonal | Rabbit | 1:100 (ICC) | Invitrogen |
| FAK | IgG | Monoclonal | Mouse | 1:100 (ICC) | Millipore |
| GFP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | AbCam |
| GFP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | Life Technologies |
| GFAP | IgG | Polyclonal | Rabbit | 1:500 (ICC) | DAKO |
| GFAP | IgG | Monoclonal | Mouse | 1:300 (ICC) | Sigma |
| GFAP | IgG | Monoclonal | Chicken | 1:2000 (ICC) | AbCam |
| p75NTR | IgG | Monoclonal | Human | 1:50 (ICC) | Millipore |
| p75NTR | IgG | Monoclonal | Mouse | 1:1,000 (ICC) | Millipore |
| Aggrecan (Cat 301) | IgG | Monoclonal | Mouse | 1:300 (ICC) 1:500 (WB) | Millipore |
| Aggrecan (AB1031) | IgG | Polyclonal | Rabbit | 1:300 (ICC) 1:500 (WB) | Millipore |
| Ki67 (PP-67) | IgG | Monoclonal | Mouse | 1:200 (ICC) | AbCam |
| MBP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | AbCam |
| MBP (G7G) | IgG | Monoclonal | Mouse | 1:2000 (ICC) | AbCam |
| S100 | IgG | Monoclonal | Mouse | 1:100 (ICC) | ThermoFisher Scientific |
| Hoechst-33342 | 1:10,000 (ICC) | Sigma | |||
| Dappi (in ProLong Gold mounting medium) | ThermoFisher Scientific |