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. 2020 Jul 9;10:11264. doi: 10.1038/s41598-020-68070-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Normalized UV–Vis spectra of the raw N. maculosa eggshell extract (green trace, EtOAc), extracted biliverdin 2^Me (blue trace, MeOH), and extracted uroerythrin 3^Me (red trace, MeOH). (b) HPLC traces of the raw extract from purple N. maculosa eggshells at two different wavelengths of detection. (c) Normalized UV–Vis spectra of the raw E. elegans eggshell extract (green trace, EtOAc), and of the two major components: biliverdin 2^Me (blue trace, in MeOH) and bilirubin 4^Me (orange trace, in MeOH). (d) NP-HPLC traces (at 400 nm detection wavelength) of the polar and nonpolar fractions from the E. elegans extract.