Fig. 6. ROS regulates stability of IκB-ζ protein.

a Chondrocytes were co-treated with IL-1β (10 ng/mL) and N-acetylcysteine (3 mM) for 24 h. Western blotting was performed for IκB-ζ. b, c Gene expression analysis for IL-6 and MMP13 was performed by qPCR. Results are representative of two out of three independent experiments, due to large biological variation in third experiment with similar trends. Bars represent mean of n = 2 independent experiments. d Chondrocytes were treated with increasing concentrations of Hydrogen peroxide in the presence or absence of IL-1β for 24 h. Western blotting was performed for IκB-ζ. Image representative of one of three experiments. e, f Gene expression of IL-6 and MMP13 was measured by qPCR. Results are representative of one experiment out of three displaying similar trends but with large biological variations in gene expression. Bars represent mean of technical replicates from one experiment. g Chondrocytes were then treated with FX11 (40 μM) in the presence or absence of H₂O₂ (50 μM) and IL-1β (10 ng/mL) for 24 h. Western blotting was performed for IκB-ζ. Image representative of one of two experiments.