Fig. 7. Deletion of LDHA in chondrocytes in vivo is protective against OA.
a MLI surgery was performed on 10-week-old AcanΔ/Ldha male mice placed on tamoxifen diet to induce recombination. Sham surgery was performed on contralateral leg and used as control. 10 weeks post surgery mice were sacrificed and joints were collected for histology. Safranin-O staining was performed of the MLI and sham joints. b OARSI scoring of two safranin-O stained sections per mouse were averaged (n = 3 mice for control, n = 3 mice for AcanΔ/Ldha). Bars represents mean ± S.D. from one experiment out of two comparing littermates. One-way ANOVA followed by Tukey’s test multiple comparisons test was utilized for statistical analysis. c IHC was performed for MMP13, with arrows pointing to examples of stained chondrocytes. Representative images are displayed with pixel intensity of stain quantified under each image. a, c Scale bar = 200 μm.