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. 2020 Jul 9;10:11339. doi: 10.1038/s41598-020-68339-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap based on the qRT-PCR analysis of the differentially expressed Tlr/MyD88-dependent and Nf-κB pathway and immuno-related genes at different times of exposure to LPS (1–72 h). Time course of gene expression in the pharynx of C. robusta exposed to LPS compared with the gene expression in untreated ascidians. Values are presented as the means ± SD. Statistical differences were estimated by one-way ANOVA and Tukey's t-test. The level of significance was set at a p-value ≤ 0.05 (N = 4).