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. 2020 Jul 9;10:11404. doi: 10.1038/s41598-020-68223-8

Figure 4.

Figure 4

Cardiac PW1+CD51+ cells express a fibrogenic program. (A) Hierarchical clustering based on the expression of Peg3, Itgav, and known cell type markers in FACS-isolated PW1+ cells, cardiomyocytes, and non-myocyte fractions from normal mouse hearts. (B) Hierarchical clustering showing high expression of collagen genes in PW1+ cells. (C) Expression of Peg3 (yellow) and Itgav (blue) in major cardiac cell populations identified after unsupervised clustering of single-cell RNA-sequencing data as previously reported25. Each point depicts a single cell. Principal component analysis and t-distributed stochastic neighbor embedding (t-SNE) were performed to provide a 2D mapping of clusters. Joint expression of Peg3 and Itgav within single cells is showed in purple. (D) The abundance of Peg3 and Itgav mRNAs and those encoding Itgβ1, Itgβ3, Itgβ5, and Itgβ8 according to cell types identified using single-cell RNA-sequencing from normal mouse hearts. (E) Western blots for ITGβ1, ITGβ3, ITGβ5, and ITGβ8 in PW1+ versus PW1 cardiac fractions. Proteins from HUVEC cells and neonatal mouse heart were used as positive controls. Ponceau Red staining showed equal protein loading.