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. 2020 Jun 3;25(7):548–557. doi: 10.1007/s10495-020-01613-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig.4 — Analysis of RIP3 expression. Live cells expressing RIP3 but not caspase-3 were analysed for RIP3 expression for untreated cells, then average percentage in RIP3 MFI ± SD were determined for 0.5 μM shikonin (24 h), 0.5 μM shikonin with 20 μM zVAD or 0.5 μM shikonin with 60 μM Nec-1. Average percentage of RIP3 MFI ± SD (n = 3), were analysed for significance by One Way ANOVA (P < 0.5) and post ad hoc tested for significance, NS (P = > 0.5, not significant), significance was P < 0.05*, P < 0.01**, P < 0.001***, P < 0.0001**** compared to untreated cells