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. 2020 Jun 10;21:394–411. doi: 10.1016/j.omtn.2020.06.005

Figure 3.

Figure 3

METTL3 Overexpression Promotes Ang II-Infused AAA Formation

(A) Western blots showing the expression of METTL3 over time after AAV-METTL3 transfection in the suprarenal aortas of Ang II-treated C57BL/6J mice (n = 3). (B) Two-dimensional color-coded ultrasound imaging of AAAs after 14 days of Ang II treatment. (C) Representative photographs of the macroscopic features of AAAs in Ang II-infused C57BL/6J mice. (D) Statistical analysis of AAA incidence in Ang II-infused C57BL/6J mice. (E) Maximal aortic diameters in Ang II-infused C57BL/6J mice. (F) Representative elastin staining and elastin degradation scores in suprarenal aortas from Ang II-infused C57BL/6J mice. The magnified photographs were taken at the position where the most severe elastin degradation occurred (scale bars, 200 and 50 μm; magnified photographs). The data are presented as the medians and quartiles. ∗∗p < 0.01. (G) Representative immunostaining for MAC2 (scale bars, 200 and 50 μm) and corresponding densitometric analysis (n = 4). (H and I) Western blots (H) and densitometric analysis (I) of the protein expression of METTL3, P21, MMP2, and MCP1 in Ang II-induced male C57BL/6J mice (n = 4). (J) Representative in situ zymography photographs of MMP activity in Ang II-induced male C57BL/6J mice (scale bars, 50 μm; n = 4). The data are presented as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01.