Figure 7.

miR34a Promotes AAA by Inhibiting SIRT1

(A) Representative photographs of the macroscopic features of AAAs in Ang II-infused DMSO- or EX527-treated C57BL/6J mice after anti-miR34a virus infection. (B) Statistical analysis of AAA incidence in Ang II-infused DMSO- or EX527-treated C57BL/6J mice. (C) Maximal aortic diameters in the Ang II-infused C57BL/6J mice in the two groups. (D and F) Representative elastin staining (D) and elastin degradation scores (F) in suprarenal aortas from Ang II-infused C57BL/6J mice. The magnified photographs were taken at the position where the most severe elastin degradation occurred (scale bars, 200 and 50 μm; magnified photographs). (E and G) Representative immunostaining for MAC2 (E) (scale bars, 200 and 50 μm) and the corresponding densitometric analysis (G) (n = 4). (H–K) Representative immunostaining and densitometric analysis for MMP2 (H and I) and MCP1 (J and K) (scale bars, 200 and 50 μm; n = 4). (L) Relative mRNA expression of MMP2, MCP1, and P21 in Ang II-infused DMSO- or EX527-treated C57BL/6J mouse aortas. (n = 4). The data are presented as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01.