Figure 3.

The effects of microRNA inhibitor on COTL1 expression and cell invasion ability. (A) MiR-30c-5p expression levels were determined by RT-PCR after cells were transfected with miR-30c-5p inhibitor, with U6 being used for the loading control. (B) mRNA and protein expression levels were determined by RT-PCR and Western blotting after cells were transfected with miR-30c-5p inhibitor. The data are presented as mean ± SE. GAPDH was employed as the loading control. (C) Cell invasion ability was assessed by Transwell assay after cells were transfected with miR-30c-5p inhibitor. *, P<0.05; *** means P<0.001.