Table 1.
List of antibodies.
| Antibody | Source | Identifier | Dilution | |
|---|---|---|---|---|
| 1 | Mouse-anti-AIB1 | BD transduction laboratories | 611,105 | 1:1,000 |
| 2 | Rabbit-anti-SRC-3 (5E11) | Cell signalling Technology | 2,126 |
1:1,000 (WB) 1:200 (immunostaining) |
| 3 | Rabbit-anti-NCoA-3 (M-397) | Santa Cruz Biotechnology | sc-9119 | – |
| 4 | Rabbit-anti-ERK 2 (C-14) | Santa Cruz Biotechnology | sc-154 | 1:1,000 |
| 5 | Mouse-anti-phospho p44/42 MAPK (ERK1/2) (T202 Y204) | Cell Signaling Technology | 9,106 | 1:1,000 |
| 7 | Rabbit-anti-MK2 | Cell Signaling Technology | 3,042 | 1:1,000 |
| 8 | Rabbit-anti-phospho-p38 MAPK (T180/Y182) | Cell Signaling Technology | 9,211 | 1:1,000 |
| 9 | Rabbit-anti-phospho-MK2 (T334) | Cell Signaling Technology | 3,041 | 1:1,000 |
| 10 | Rabbit-anti-p38 MAPK | Cell Signaling Technology | 9,212 | 1:1,000 |
| 11 | Mouse-anti-FLAG | Sigma-Aldrich | F1804 | 1:1,000 |
| 12 | Rabbit-anti-actin | Sigma-Aldrich | A2066 | 1:1,000 |
| 13 | Rabbit-anti-GST (Z-5) | Santa Cruz Biotechnology | sc-459 | 1:1,000 |
| 14 | Mouse-anti-PRAK (A-7) (MK5) | Santa Cruz Biotechnology | sc-46667 | 1:1,000 |
| 15 | Mouse-anti-MAPK6 (ERK3) | Abnova | H00005597-M02 | 1:1,000 |
| 17 | Sheep-anti-P-SRC-3-S857 | Custom made by Division of Signal Transduction Therapy, (DSTT), University of Dundee, Dundee, UK | 1:1,000 | |
| 18 | Goat-anti-mouse AF 800 | Invitrogen | A32730 | 1:10,000 |
| 19 | Goat-anti-mouse AF 700 | Invitrogen | A21036 | 1:10,000 |
| 20 | Goat-anti-rabbit AF 800 | Invitrogen | A32735 | 1:10,000 |
| 21 | Goat-anti-rabbit AF-700 | Invitrogen | A21038 | 1:10,000 |
| 22 | Rabbit-anti-sheep DyLight 800 | Invitrogen | SA5-10060 | 1:10,000 |
| 23 | Donkey-anti-sheep AF 680 | Invitrogen | A-21102 | 1:10,000 |
| 24 | Donkey-anti-rabbit AF 568 | Invitrogen | A-10042 | 1:4,000 |
| 25 | Mouse-anti-IKK-α (B-8) | Santa Cruz Biotechnology | sc-7606 | 1:1,000 |
| 26 | Rabbit-anti-IκB-α (C-21) | Santa Cruz Biotechnology | sc-371 | 1:1,000 |
| 27 | Mouse-anti-HSP27 | Millipore | MAB88051 | 1:1,000 |
| 28 | Rabbit anti-phospho-HSP27 (S82) | Cell Signaling Technology | 2,401 | 1:1,000 |
BD transduction laboratories, NJ, USA; Santa Cruz Biotechnology, CA, USA; Cell Signaling Technology, Danvers, MA, USA; Abnova, Taipei City, Taiwan; Invitrogen, CA, USA, Sigma-Aldrich. If not indicated, the antibody was used for Western-blotting (WB).