Table 2.
List of oligonucleotides.
| Target gene | Primer | Purpose | Nucleotide sequences | Source |
|---|---|---|---|---|
| IL-6 | F | RT qPCR | GCAGAAAAAGGCAAAGAATC | Sigma-Aldrich |
| R | CTACATTTGCCGAAGAGC | |||
| IL-8 | F | RT qPCR | GTTTTTGAAGAGGGCTGAG | Sigma-Aldrich |
| R | TTTGCTTGAAGTTTCACTGG | |||
| MMP9 | F | RT qPCR | AAGGATGGGAAGTACTGG | Sigma-Aldrich |
| R | GCCCAGAGAAGAAGAAAAG | |||
| TRAF1 | F | RT qPCR | CTTTCCTGTGGAAGATCAC | Sigma-Aldrich |
| R | ACTTGGCAGTGTAGAAGG | |||
| ICAM1 | F | RT qPCR | ACCATCTACAGCTTTCCG | Sigma-Aldrich |
| R | TCACACTTCACTGTCACC | |||
| GAPDH | F | RT qPCR | CTTTTGCGTCGCCAG | Sigma-Aldrich |
| R | TTGATGGCAACAATATCCAC | |||
| TFRC | F | RT qPCR | AAGATTCAGGTCAAAGACAG | Sigma-Aldrich |
| R | CTTACTATACGCCACATAACC | |||
| IL-6-prom | F | Promoter PCR | CCGGGTACCTCCAAGGCAGACTCTGAG | Sigma-Aldrich |
| R | GGCCAAGCTTCATCTCCAGTCCTATATTTATTGGGGG | |||
| M13 | R | PCR | CAGGAAACAGCTATGAC | Sigma-Aldrich |
| SRC-3-A | F | PCR | AGGAAGGGGAAGGTAAGAGCTA | Sigma-Aldrich |
| R | CACAGGGTTTGATGGAAATGTT | |||
| SRC-3-B | F | CRISPR sgRNA | GCAATCTTGTATGATCTGTG | Life Technologies |
| R | CACAGATCATACAAGATTGC | |||
| SRC-3-C | F | siRNA | CAGUAUAUCGAUUCUCGUUtt | Ambion life technologies |
| R | AACGAGAAUCGAUAUACUGgg | |||
| SRC-3siR | F | Mutagenesis | CCATGCAGAAACCCCCGTCTACCGCTTCTCGTTGGCTGAT | Sigma Aldrich |
| R | ATCAGCCAACGAGAAGCGGTAGACGGGGGTTTCTGCATGG | |||
| IKK-α | F | siRNA | GAAGGAUCCAAAGUGUAUAtt | ThermoFisher Scientific |
| UAUACACUUUGGAUCCUUCgg |
(Life Technologies, CA, USA) (Ambion life technologies, Carlsbad, CA, USA).
The same siRNA against ERK3, MK5 and scrambled siRNA were used as reported in25.