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. 2020 Jul 9;11:3501. doi: 10.1038/s41467-020-17093-9

Fig. 5. ASOs increase protein levels in a dose-dependent manner in vitro.

Fig. 5

a Bar graph plotting the quantification of PCCA western blot analysis from HEK293 cells treated with hit ASO at increasing concentrations for 48 h. Equal protein loading was confirmed with Ponceau staining (Supplementary Fig. 5). b Same for SynGAP (p = 4.62e-6). c Bar graph plotting flow cytometry derived fold change of the Mean Fluorescent Intensity of PD-L1 from Huh7 cells treated with the hit ASO at increasing concentrations for 120 h. No-ASO (−), scramble (SC), and mismatch (MM) controls were included in each experiment at the same increasing concentrations as the respective targeting ASOs (p = 7.70e-5). All experiments were performed in three biological replicates. Data are presented as mean values ±SD. P-values were calculated based on two-sided t-test. Asterisks indicate p < 0.001. Source data are provided as a Source Data file.