Table 3.
Receiver operating characteristics (ROC) curves and Youden Index cut-offs of Anisakis-sIgE (InmunoCAP), rAni s 1 (ELISA) and rAni s 7 (ELISA). (a) Comparison of receiver operating characteristics (ROC) curves of Anisakis-sIgE (InmunoCAP), rAni s 1 (ELISA) and rAni s 7 (ELISA). (b) Comparison of Youden index cut-offs of Anisakis-sIgE (InmunoCAP), rAni s 1 (ELISA) and rAni s 7 (ELISA).
| Anisakis-sIgE ~ rAni s 1 | Anisakis-sIgE ~ rAni s 7 | rAni s 1 ~ rAni s 7 | |
|---|---|---|---|
| (a) Comparison of ROC data | |||
| Difference between areas | 0.217 | 0.214 | 0.00355 |
| Standard Errora | 0.0440 | 0.0403 | 0.0503 |
| 95% confidence interval | 0.131 to 0.304 | 0.135 to 0.293 | − 0.0951 to 0.102 |
| z statistic | 4.946 | 5.304 | 0.0705 |
| Significance level | p < 0.0001 | p < 0.0001 | p = 0.9438 |
| Anisakis-sIgE | rAni s1 | rAni s 7 | |
|---|---|---|---|
| (b) Youden Index | |||
| Youden index J | 0.6437 | 0.3798 | 0.3381 |
| 95% Confidence intervalc | 0.4689 to 0.7471 | 0.1996 to 0.5035 | 0.1603 to 0.4725 |
| Associated criterion | > 7.9 | > 1.464 | > 0.589 |
| 95% Confidence intervalc | > 1.8 to> 26.6 | > 1.34 to > 1.576 | > 0.053 to> 1.165 |
| Sensitivity | 79.59 | 51.02 | 81.63 |
| Specificity | 84.78 | 86.96 | 52.17 |
The sample was defined as positive group (n = 49; 51,58%), patients or blood donors with symptoms related to fish intake; and as negative group (n = 46; 48,42%), sensitized blood donor without symptoms after eating fish. Cut-off values: Anisakis-sIgE > 0.35 kUA/L; SPT ≥ 3 mm; rAni s 1 = 0.09 (OD); rAni s 7 = 0.05 (OD). aDeLong et al.51, bbinomial exact. cBCa bootstrap confidence interval (1,000 iterations; random number seed: 978). OD, optical density.