Fig. 4. Analysis of αSynO and αSynO−huPrP(23−144) clusters.

a–h AFM data are shown both as raw height images (a–d) and after edge detection using the Sobel operator for visualization of structural details (e–h). AFM scale bars represent 500 nm. i–k TIRFM shows the presence of clusters in a sample containing both 10 µM αSynO and 2 µM huPrP(23−144), 10% AlexaFluor488-labeled. l DLS measurement of heteroassemblies generated from 10 µM αSynO and 20 µM huPrP(23−144) and isolated by sucrose DGC.