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. 2020 Jul 3;11:996. doi: 10.3389/fpls.2020.00996

Figure 1.

Figure 1

Targets locations and sequence alignments. (A) Structures of PtPDS genes in P. trichocarpa. T1 and T2 indicate the targets locations. F1 and R1 were used to amplify Shanxin yang PDS genes fragments. F2 and R2 were used to amplify the target region. (B) PCR amplification of PdbPDS genes fragments. (C) Alignment of two PdbPDS1 allele fragments and Potri.014G148700. (D) Alignment of one PdbPDS2 allele fragment and Potri.002G235200. In (C, D), the green lines below aligned sequences indicate predicted exons within the amplified fragments; and the blue and pink boxes represent potential target sites that may be bind by sgRNA1 and sgRNA2, respectively. The first number in each target name represents the gene number, while the second number represents the number of sgRNA, e.g., T1-1 means the target site in PdbPDS1 that can be bounded by sgRNA1.