Table 3.

Summary of the mutation types at each target site.

Mutation type	No. of plants
	Targeted by sgRNA1		Targeted by sgRNA2
	T1-1	T2-1	T1-2	T2-2
Insertion only	0	1	0	0
Deletion only	40	40	19	2
Insertion and deletion	15	4	0	0
Subsitution	0	0	1	0
Homozygous mutation	9	25	1	0
Heterozygous mutation	3	9	19	2
Biallelic mutation	33	9	0	0
Chimeric mutation	10	2	0	0
Plants with mutation	55	45	20	2
Unmodified	7	17	42	60
Mutation rate	88.7%	72.5%	32.3%	3.2%