Table 1.

De Novo Motif Analysis of β-Catenin-Associated Cis-regulatory Sequences

A: De Novo Motif Analysis First Surge Genes					B: De Novo Motif Analysis Second Surge Genes
Rank	Discovered Motif	Best Match	p Value	%	Rank	Discovered Motif	Best Match	p Value	%
1		TCF7L2	110−20	26	1		FOXH1	1 × 10−99	49
2		FOXH1	1 × 10−19	32	2		Tbx21-like T-box	1 × 10−41	14
3		LEF1	1 × 10−18	35	3		Helix-turnhelix (homeobox?)	1 × 10−28	5
4		Pan/dTCF	1 × 10−18	23	4		TCF7L2	1 × 10−28	18
5		Pan/dTCF	1 × 10−18	16	5		C2H2 zinc finger	1 × 10−26	4
6		C4 zinc finger (GATA?)	1 × 10−16	16	6		ROX1-like HMGbox	1 × 10−25	7
7		Zinc finger	1 × 10−15	23	7		NFkB-like	1 × 10−25	42
8		SOX	1 × 10−14	13	8		TDA9-like zinc finger	1 × 10−24	6
9		Meis1	1 × 10−13	42	9		MBP1-like helix-turnhelix	1 × 10−22	15
10		TOD6-like	1 × 10−13	26	10		NHP10-like HMG box	1 × 10−14	7

De novo motif analysis of sequences surrounding β-catenin-associated locations (at early blastula st.7) in maternal Wnt/β-catenin-regulated genes at the onset of zygotic transcription (ZGA) (Table 1A, mid-blastula st.8, note Tcf/Lef and Foxh1 consensus motifs) and approximately 1 h later (Table 1B, late blastula st. 9, note Foxh1 and Tcf/Lef consensus binding motifs).