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. 2020 Jul 3;13:120. doi: 10.3389/fnmol.2020.00120

Figure 4.

Figure 4

CD4+ cells modulate astrocyte ability to affect endothelial properties. Astrocytes (AC) were pre-exposed to Aβ (2.5 μM) for 5 h and then co-cultured with CD4+ cells (AC/CD4). Astrocytic mRNA levels of IL-6 (A), IL-1β (B), and VEGF (C) were evaluated by RT-PCR. Endothelial cells were co-cultured with naïve astrocytes (AC) or astrocytes co-cultured with CD4+ cells (AC/CD4) and treated with Aβ (2.5 μM) for 18 h. Protein expression of claudin-5 and ICAM-1 was evaluated by western blot analysis. A representative blot and densitometric analysis of claudin-5 (D) and the two ICAM-1 isoforms (85 and 75 kDa) are reported (E). Data are mean ± SEM of three (A–C), four (D), and six (E) independent experiments. *p < 0.05 vs. control, ctr. $p < 0.05 vs. the condition without CD4 (AC). Significance was assessed by one-way ANOVA followed by Newman–Keuls test.