Fig. 2.

Combined CB-839/anti-EGFR mAb therapy results in cooperative efficacy in 2D culture in vitro. CRC cell lines were propagated in 2D culture. Cells were treated with either cetuximab (black), CB-839 (white), or cetuximab and CB-839 (grey). Cell viability was determined using MultiTox Glo cytotoxicity assay. Shown is the percentage change in live cells from the fluorescence assay normalized to vehicle control. CRC cell lines propagated in 2D culture exhibit reduced viability with combined therapy compared to single agents (relative to vehicle control). Error bars represent ± standard deviation (SD) (n = 3 or 4 technical replicates). Combination indices were determined and are indicated for each cell line by the following symbols: * synergism, ^ additive, and” not available.