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. 2020 Jul 10;20:493. doi: 10.1186/s12879-020-05220-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Extracellular a IFN-γ and b IL-4 levels elicited by hexavalent recombinant protein mixture (+) Montanide ISA 50 V and control groups. The red bars represent IFN-γ response and the green bars, IL-4 response. Each bar represents the mean ± SD value of IFN-γ and IL-4 responses of 3 mice from each group. Each bar represents the mean ± SD value of IFN-γ and IL-4 responses of 3 mice from each group. Single cell suspensions were stimulated with rH2, rA4, rE4, rD6, rE6, and rH6 recombinant proteins with a final concentration of 100 μg/ml. In figure, *** represent P ≤ 0.001 and * represent P ≤ 0.05